Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH FC

Prohibitin (PHB) proteins have been ascribed various functions, including cell cycle regulation, apoptosis, assembly of mitochondrial respiratory chain enzymes, and aging. It has been shown that the mammalian prohibitins are present in the inner mitochondrial membrane and are always bound to each other, with no free protein detectable. They are coexpressed during development and in adult mammalian tissues, and expression levels are indicative of a role in mitochondrial metabolism, but are not compatible with roles in the regulation of cellular proliferation or apoptosis. Data support conserved roles for the prohibitins in regulating mitochondrial respiratory activity and in aging (1). Results suggest that prohibitin may be a tumor suppressor and is associated with tumor development and/or progression of at least some breast cancers (2).
Catalog #	P9007-52
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:10,000-1:20,000
	Immunohistochemistry: 1:250-1:500
	Flow Cytometry: 1:50
	Immunoprecipitation: 1:60
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E472
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues on the N-terminus of human prohibitin.
Specificity	Recognizes human Prohibitin, NT.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.