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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B

Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37kD, ubiquitous member of the prohibitin family of molecules. PHB2 is found in multiple intracellular compartments. It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER). Human PHB2 is 299 amino acids (aa) in length. It contains a mitochondrial localization signal (aa 1-50), two ER repressor domains (aa 19-49 and aa 150-174), and a central coiled-coil domain (aa 190-238). There may be one splice variant that shows a premature truncation after aa 289. Over aa 89-299, mouse and rat PHB2 are absolutely (100%) identical to human PHB2.
Catalog #	P9007-53
Applications	Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Direct ELISA: 0.5-1.0ug/ml detection limit for rhPHB2 is ~ 6.5ng/well.
	Western Blot: 1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	E. coli-derived rhPHB2. (aa 89 - 299)
Specificity	Recognizes human/mouse/rat PHB2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.