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You are here:Home » Antibodies » Antibodies-Insulin » Anti -Proinsulin

Anti -Proinsulin


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations. The antibody recognizes insulin and recombinant proinsulin, but does not react with synthetic C-peptide.
Catalog #P9008-06F
ApplicationsSuitable for use in Indirect ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Indirect ELISA: 2ug/ml
Immunohistochemistry: 20-40ug/ml
Western Blot: 4-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No10g81
FormSupplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant full length Insulin expressed in yeast
SpecificityRecognizes Proinsulin. Species crossreactivity: human, bovine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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