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You are here:Home » Biochemicals » Biochem-Molecular Biology » Propidium Iodide

Propidium Iodide


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Propidium iodide (PI) is a fluorescent dye [FW 668.41] that binds specifically to DNA. This property has led to its common use in evaluation of cell cycle, aneuploidy and apoptosis by flow cytometry. When excited by a laser light at 488 nm, PI emits a signal that can be monitored by the red wavelength detector typically reserved for phycoerythrin (usually FL2). Propidium iodide is excluded from viable cells, thus analysis of DNA content in cells requires membrane permeabilization. In evaluation of apoptosis, PI is used to distinguish necrotic cells or cells in late stages of apoptosis, which have permeabilized membranes and thus bind PI, from viable cells or cells in early apoptosis where membranes are intact and PI is excluded
Catalog #P9052-05
Used in conjunction with the United States Biological Annexin V Apoptosis Detection Kits (A2296-10, -20, -30).
Solubility Soluble in water (1mg/ml), DMSO, and methanol (3mg/ml)
Appearance (1mg/ml, H2O): Very dark red to red, clear, complete
Water (KF): 5.0%
Identity (IR): As Reported
Melting Point: 220-225°C
Fluorescence: ex 536nm, em 617nm
pKa: 1.63
CAS Number25535-16-4
Molecular FormulaC27H34N4I2
Molecular Weight668.4
FormVery dark red to red powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide

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