Gene Ontology Terms: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; electron transporter activity; metabolism; oxidoreductase activity; prostaglandin metabolism; prostaglandin-D synthase activity

Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: experiments gave bands at approx 26kD and 50kD in Human Duodenum lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature (PMID: 11889207). This protein has a calculated MW of 29.0kD according to NP_000851. Recommended concentration: 0.1-1ug/ml.

0.1ug/ml staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Corresponding peptide is available for Peptide Blocking studies. See P9053-27A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

C-DYDTTPFQAKTQ, C Terminus

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purified by immunoaffinity chromatography.

Recognizes Human, Chimpanzee Prostaglandin dehydrogenase 1