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You are here:Home » Antibodies » Abs to Prostaglandins » Anti -Prostaglandin Transporter (PGT)

Anti -Prostaglandin Transporter (PGT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called “renal secretion”. Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Prostaglandins and thromboxanes play important physiological, pathological, and therapeutical roles in health and disease. PGT prostaglandin transporter also known as martin F/G), a member of oatp family, mediates transport of PGs (PGD2, PGE1, PGE2, PGF2a) from the circulation. PGT (rat/human 643 aa; 12 TM) is
expressed in brain, stomach, ileum, jejunum, and kidney but not in heart or skeletal muscle.
Catalog #P9053-52
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
Immunogen20aa peptide from the C-terminus region of Rat PGT (KLH).
SpecificityRecognizes rat PGT. No significant sequence homology is detected with other oatp1-3, OATP or LST-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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