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You are here:Home » Culture Media » Media-Cell Culture, Serum Free » Prostate Cell, Immortalized Normal, Serum Free Media (BRFF P4-8F)

Prostate Cell, Immortalized Normal, Serum Free Media (BRFF P4-8F)

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Specifications

BRFF-P4-8F was designed to grow the immortalized normal prostatic cell line 267-B1. It also supports the growth of certain established human prostatic cancer cell lines such as PC-3. For optimum cell attachment, use FNC Coating Mix®.
Ready-to-use with all necessary growth factors. Antibiotic-free.
Catalog #P9054-01
StorageStore at -70°C. Supplied frozen.
Stability at 4°C4-6 weeks
pH7.2-7.5 (5% CO2 atmosphere is recommended for culture.)
Osmolality270-300mOsM
Sterility TestSpecification: Detection of microbial growth.
Test Sample: 0.1ml into each of the following media (in triplicate)
Incubation Conditions: 37°C/48 hrs
Functional TestSpecification: Growth of cell line 276-B1 to confluence. One vial containing 1-5x10e6 cells is quick thawed, washed with 10ml of medium, suspended in 10ml of medium and applied to a T-25 flask pre-coated with FNC Coating Mix®. The culture is incubated at 37°C in a 5.0% CO2 incubator.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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