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You are here:Home » Antibodies » Antibodies-Proteins » Anti -Prosurfactant Protein B (proSP-B)

Anti -Prosurfactant Protein B (proSP-B)


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. It is composed primarily of phospholipids (phosphatidylcholine and phosphatidylglycerol) and lesser amounts of cholesterol and surfactant-associated protein. Prosurfactant Protein B is a precursor of a very hydrophobic protein of the surfactant.
Catalog #P9054-86
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:4000-1:8000 (Human tissue with and without antigen retrieval)
Immunohistochemistry: 1:1000-1:2000 (Adult and fetal mouse lung)
Western Blot: 1:1000-1:5000
ELISA: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot Determined
FormSupplied as a liquid, 0.01% sodium azide.
ImmunogenFull-length recombinant peptide (381aa) corresponding to human SP-B (pre)proprotein.
SpecificityRecognizes human proSP-B (detects both the N-terminal and C-terminal propeptides). Does not crossreact with mature SP-B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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