| Catalog # | P9070 |
| Applications | Used generally to inhibit proteases (serine, cysteine, aspartic and metalloproteases). |
| One bottle is suf cient to inhibit proteases present in 20g of cellular extract. Note that some organisms contain higher levels of proteases and may require a higher concentration of protease cocktail to inhibit the intrinsic proteolytic activity. |
| Activity | One ml of the cocktail will inhibit proteolytic activity equivalent to 1mg of USP pancreatin. |
| Inhibitor Targets | AEBSF: Serine proteases, such as trypsin and chymotrypsin |
| EDTA Metalloproteases |
| Bestatin: Aminopeptidases, such as leucine aminopeptidase and alanyl aminopeptidase |
| E-64: Cysteine and thiol proteases, such as calpain, papain, cathepsin B and cathespin L |
| Leupeptin: Serine and thiol proteases, such as calpain, trypsin, papain and cathepsin B Aprotinin: Serine proteases such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase and kallikrein. Inhibits leukocyte elastase but not pancreatic elastase |
| Reconstitution | Dissolve powder in 10ml of RIPA lysis buffer (10X) and transfer to a larger container to prepare 100ml final volume. The powder may be prepared and stored as a more concentrated stock for further dilution. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 1 week at 4ºC. |
| Form | Supplied as lyophilized powder containing 2mM AEBSF, 1mM EDTA, 130uM Bestatin, 14uM E-64, 1uM Leupeptin, 0.3uM Aprotinin when reconstituted in 100ml of buffer. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
