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You are here:Home » Antibodies » Abs to Proteasomes » Anti -PSME3 (Proteasome Activator Complex Subunit 3, Proteasome Activator 28 Subunit gamma, PA28gamma, PA28g, Activator of Multicatalytic Protease Subunit 3, 11S Regulator Complex Subunit gamma, REG-gamma, Ki Nuclear Autoantigen)

Anti -PSME3 (Proteasome Activator Complex Subunit 3, Proteasome Activator 28 Subunit
gamma, PA28gamma, PA28g, Activator of Multicatalytic Protease Subunit 3, 11S Regulator
Complex Subunit gamma, REG-gamma, Ki Nuclear Autoantigen)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome,is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified.
Catalog #P9075-85A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in lysates of cell lines Jurkat and K562 on ~30kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-KNIEKIKRPRSSN, from human PSME3, at C-terminal (NP_005780.2, NP_789839.1).
SpecificityRecognizes human PSME3. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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