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You are here:Home » Antibodies » Abs to Proteasomes » Anti -Proteasome alpha 3 (PSMA3)

Anti -Proteasome alpha 3 (PSMA3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with 7 distinct subunits. The two outer layers are identical rings composed of subunits (called PSMAs), and the two inner layers are identical rings composed of subunits. While the catalytic sites are located on the rings (1-3), the subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different and ten different proteasome genes have been identified in mammals (5).PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome which preferentially degrades poly-ubiquitinylated proteins (1-3). The proteasome has a broad substrate spectrum which includes cell cycle regulators, signaling molecules, tumor suppressors and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune response, protein folding and disease progression (6-9).
Catalog #P9076-05
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic peptide (KLH-coupled) corresponding to residues surrounding Val228 of human PSMA3 protein.
SpecificityDetects endogenous levels of total human PSMA3 protein. Species Crossreactivity: Monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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