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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IF

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with 7 distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5). PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome which preferentially degrades poly-ubiquitinylated proteins (1-3). The proteasome has a broad substrate spectrum which includes cell cycle regulators, signaling molecules, tumor suppressors and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune response, protein folding and disease progression (6-9).
Catalog #	P9076-07
Applications	Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunofluorescence (IF-IC): 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.15M sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A and immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to residues surrounding Lys231 of human PSMA5 protein (KLH).
Specificity	Recognizes endogenous levels of total human PSMA5 protein. Species crossreactivity: mouse, rat and monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.