Anti -Proteasome 20S alpha, beta (30kD Prosomal Protein, HC2, Macropain, Macropain Subunit
nu, Macropain Subunit zeta, Macropain zeta Chain, MGC117302, MGC125802, MGC125803,
MGC125804, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, Multica
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IP IH |
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| The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kD, 450A in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. | | | Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha-subunits comprise the outer rings and the beta-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha 7 beta 7 beta 7 alpha 7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the alpha- or beta-rings (1). | | | 20S Proteasomes degrade only unfolded proteins in an energy-independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP-dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome (2). | | | Catalog # | P9078-38B | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000-1:10,000 | | Immunohistochemistry: Paraffin | | Optimal dilutions to be determined by the researcher. | | Positive Control | Human and yeast 20S proteasome and human placental proteasome preps | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, 10mM sodium azide. | | Purity | Serum | | Immunogen | A proteasomal preparation isolated from human red blood cells. Cellular Localization: Cytoplasmic and Nuclear | | Specificity | Recognizes a combination of human alpha and beta Proteasome subunits. Species Crossreactivity: Mouse, S. cerevisiae | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Proteasome 20S alpha, beta (30kD Prosomal Protein, HC2, Macropain, Macropain Subunit nu, Macropain Subunit zeta, Macropain zeta Chain, MGC117302, MGC125802, MGC125803, MGC125804, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, Multica |
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