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You are here:Home » Antibodies » Abs to Proteasomes » Anti -Proteasome 20S C2 (30kD Prosomal Protein, HC2, Macropain, Macropain Subunit C2, Macropain Subunit nu, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, Multicatalytic Endopeptidase Complex Subunit C2, NU, PROS30, Proteasome (Prosome, Ma

Anti -Proteasome 20S C2 (30kD Prosomal Protein, HC2, Macropain, Macropain Subunit C2,
Macropain Subunit nu, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915,
Multicatalytic Endopeptidase Complex Subunit C2, NU, PROS30, Proteasome (Prosome, Ma

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in, cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5MD. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF trans- membrane regulator), Angelman's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).
Catalog #P9078-38C1
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml detects a 29kD protein representing Proteasome 20S C2 subunit from rat brain extract.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa249-263, CP(249)ADEPAEKADEPMEH(263), from the C-terminus of human proteasome 20S C2 subunit.
SpecificityRecognizes human proteasome 20S C2 subunit. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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