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Anti -Proteasome 20S Z (Proteasome Subunit Z, Macropain Chain Z, Multicatalytic Endopeptidase Complex Chain Z, Proteasome Subunit beta Type-7)
Anti -Proteasome 20S Z (Proteasome Subunit Z, Macropain Chain Z, Multicatalytic
Endopeptidase Complex Chain Z, Proteasome Subunit beta Type-7)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5MD. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman s syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels). | | | Catalog # | P9078-38Z | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic Peptide corrseponding to aa263-277 [CE(263)IEVLEETVQTMDTS(NH2)(277)] of human Proteasome 20S Z . | | Specificity | Recognizes human Proteasome 20S Z at ~29kD. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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