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You are here:Home » Antibodies » Abs to Proteasomes » Anti -Proteasome 26S, P27 Subunit

Anti -Proteasome 26S, P27 Subunit

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IF
Catalog #P9078-60
ApplicationsSuitable for use in Immunofluorescence microscopy, Western Blot, Immunoprecipitation and Affinity chromatography.
Recommended DilutionImmunofluorescence: 1:100-1:500; Incubate 15-20 min. at RT; longer incubation may result in loss of soluble antigen.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F283
HostMouse
SourceXenopus
ConcentrationNot determined
FormSupplied as a liquid containing 0.09% sodium azide.
PurityAscites
ImmunogenNuclear 26S complexes of Xenopus laevis oocytes
SpecificityAntigen Recognized in Species and Xenopus laevis; XLKE-A6 (Xenopus laevis). Polypeptide Reacting p27 subunit of 20S cylinder particles. Cultured Cell Lines (tested so far) PLC (human); RVF (rat); Ptk2 (kangaroo rat). Recognizes the 20S subcomplex within the 26S heterooligomeric protein complex and the free cytosolic form of 20S cylinder particles; the epitope is resistant to formaldehyde fixation (up to 4%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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