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You are here:Home » Antibodies » Abs to Proteasomes » Anti -Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31, Proteasome Inhibitor Subunit 1 PI3-1, Proteasome Inhibitor hP131 Subunit, PSMF1)

Anti -Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31, Proteasome Inhibitor
Subunit 1 PI3-1, Proteasome Inhibitor hP131 Subunit, PSMF1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
Catalog #P9078-81
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:32,000
Western Blot: 1-3ug/ml ~35kD band observed in Human Kidney lysates (calculated MW of 29.8kD according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35kD is observed. This band is not observed in the non-transfected HEK293.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to C-DHLPPPGYDDMYL, from the C Terminus of human PSMF1 (NP_006805.2).
SpecificityRecognizes human PSMF1 . Species sequence homology: mouse, rat, canine, bovine.
Expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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