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Purification Media, BioSeparation™ Affinity, Avidin Beads
Purification Media, BioSeparation™ Affinity, Avidin Beads
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| (Biotin-Capture) Avidin beads are useful carriers for biotinylated molecules, in various applications such as the construction of affinity columns or for conducting immuno-precipitations (IP). Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. Modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications. | | | Catalog # | P9100-05 | | Biotin-Capture Beads Specifications | Matrix: Sepharose CL-4B | | Coupling method: TargetLock | | Type of Avidin bound to beads: Modified Avidin | | Binding capacity: 2-4mg biotinilated BSA per ml of beads | | Bead size: 40-165um | | Bead structure: Highly cross-linked spherical agarose, 4% | | Max back pressure: 0.3MPa, 3 bar | | Max. flow rates: 4ml/min/cm2 | | Recommended flow rate: 1-2ml/min/cm2 | | Stability of the matrix: pH 2-11. | | Storage: 4°C in PBS pH 7.4 added with sodium azide 0.05% (w/v) as a preservative. | | Storage | Store gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible | | buffer added with 0.1% azide (w/v) as a preservative until use. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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