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You are here:Home » Molecular Biology » MB-BioSep Affinity Beads » Purification Media, Divinyl Sulfone (DVS) Beads, BioSeparation™

Purification Media, Divinyl Sulfone (DVS) Beads, BioSeparation™


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Divinyl Sulfone (DVS) Activated Beads are rigid Sepharose beads loaded with high density of reactive groups. The matrix is suitable for binding amino or hydroxyl groups, present on biomolecules such as proteins and carbohydrates. The DVS activated beads are stable for extended period of time when stored refrigerated and light-protected. Coupling reactions to biomolecules may be done between pH values of 8-10.
DVS Activated Beads Characteristics:
Catalog #P9100-15
Matrix Sepharose CL-4B
Activation Method: DivinylSulfone
Binding Capacity: 2-4mg BSA/ml.
Bead Size: 40-165um
Bead Structure: Highly cross-linked spherical agarose, 4%
Max Back Pressure: 0.3MPa ,3bar
Max.Flow Rates: 15ml/min/cm2
RecommendedFlow Rate: 8-12ml/min/cm2
Buffersrequired for protein conjugation (using10mlof DVS Activated Beads):
Scale up or down all values when conjugating more or less than 10ml of beads:
DVS Activated Beads:10ml
Double distilled water (100ml).
Neutral conjugation buffer (50ml): Phosphate Buffered Saline (PBS) pH 6-8
Conjugation buffer (50ml): 0.1N Sodium bicarbonate buffer (pH-8-10).
Storage and StabilityMay be stored at 4°C. For long-term storage, add sterile ddH20 store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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