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You are here:Home » Molecular Biology » MB-BioSep Affinity Beads » Purification Media, Epoxy Beads, BioSeparation™

Purification Media, Epoxy Beads, BioSeparation™


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Epoxy-Activated Beads is a preactivated support that contains high density of epoxy groups used for immobilization of various ligands. The preactivated support can be used to immobilize proteins, carbohydrates and various ligands via stable linkage to amino, thiol and hydroxyl groups. Coupling to hydroxyls is favored at a higher pH of 11-13, while Thiol-containing ligands are best coupled at pH 7.5-9.0.
Epoxy-Activated Beads characteristics:
Matrix: Sepharose CL-4B
Active group: Oxiran
Active group density: 10–25umole/ml
Bead size: 45-165um
Bead structure: Highly cross-linked spherical agarose, 6%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-2 ml/min/cm2
Stability of the matrix: pH 2-11
Catalog #P9100-20
StorageStore gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible
buffer added with 0.1% azide (w/v) as a preservative until use.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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