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You are here:Home » Molecular Biology » MB-BioSep Affinity Beads » Purification Media, Maleimide-OvA, BioSeparation™

Purification Media, Maleimide-OvA, BioSeparation™

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Specifications

Activated KLH, BSA and Ovalbumin (OVA), are commonly used as protein carriers for haptens such as peptides in order to enable the immune response to small molecules. In many cases KLH conjugate is used for the immunization, while BSA or OVA conjugate is used in immunoassys of the resulting antibodies. OVA conjugates are used in order to filter out the antibodies directed to the KLH. However in some cases OVA or other proteins are preffered as carrier proteins because of a variety of other reasons. The Maleimide activated KLH and OVA are preactivated with a heterobifunctional cross-linker (GMBS). These activated proteins may be reacted with biomolecules that contain a free sulfhydryl groups, to
form a stable thioether bond.
Activation method: GMBS
Catalog #P9100-60
Binding Capacity ~2-4mg of peptide (average MW of 1000-2500) per 2mg OVA
Protein Concentration 4mg/ml (0.5ml)
Storage Buffer PBS pH 7.5
Storage and StabilityStable for 3 days refrigerated
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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