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You are here:Home » BioSeparation? » BioSep-Affinity Beads » Purification Media, Glutathione Beads, BioSeparation™

Purification Media, Glutathione Beads, BioSeparation™

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Specifications

Glutathione Beads are used for the purification of glutathione S- transferase (GST) tagged proteins, produced using various commercial expression vectors. GST-fused proteins can be purified from bacterial lysates by one-step affinity purification. Glutathione Beads is state of the art affinity-resin product that consists of glutathione attached through sulfur to an epoxy-activated 4% cross-linked beaded agarose. The use of the Glutathione Beads enables the purification of various GST-fused proteins, while maintaining mild, non-denaturing conditions throughout the purification process.
Glutathione Beads characteristics:
Matrix: Sepharose CL-4B
Activation method: Oxiran
Catalog #P9100-67
Binding capacity 4-8mg/ml recombinant GST
Bead size: 40-165um
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3bar
Max. flow rate: 4ml/min/cm2
Recommended flow rate: 1-2ml/min/cm2
Stability of the matrix: pH 4-10
Storage and Stability:
Store at 4°C in PBS pH 7.4 with with sodium azide 0.1% (w/v) added as preservative.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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