You are here:Home
»
BioSeparation?
»
BioSep-Protein A/G/L
»
Purification Media, BioSeparation™ Affinity, Protein A, Recombinant
Purification Media, BioSeparation™ Affinity, Protein A, Recombinant
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Recombinant Protein-A used also in Protein A beads (P9100-75) is a genetically engineered form of Protein-A derived from Staphylococcus aureus. Non-essential regions have been modified while leaving the IgG binding sites intact. Protein A is been used extensively used for the isolation of IgG from several species of mammals. The affinity of Protein A to IgG's is different between species and IgG subclasses within a given species. A major known limitation of protein A is its weak binding to Mouse IgG1– a major immunoglobulin subclass in Mice. Despite its variable binding characteristics, Protein A is a popular choice for both antibodies binding in various assays and when coupled to beads, for Immunoprecipitation of -antigen complexes. Protein A binds specifically the Fc region of immunoglobulins leaving the Fab region free for binding the antigen. Hence, Protein A is extremely useful for isolating of immune complexes. | | | Catalog # | P9100-70 | | Protein A Beads characteristics | Source: Recombinant protein | | Molecular Weight: 23.3kD expressed in E. coli | | Purity: 90% by SDS-PAGE. | | Spectroscopic analysis: OD280nm/250nm=2.51 | | Endotoxin: 0.5EU/mg Recombinant Protein A (LAL) | | Composition: Lyophilized white Powder. 10.9mg Recombinant Protein A, 0.82 mg | | phosphate buffer salts. | | Storage before Reconstitution | Store at 4°C. Product is stable under these conditions for | | one year. | | Storage after Reconstitution | Store at 4°C in PBS pH 7.4 added with sodium azide 0.1% (w/v) as a preservative. Product is stable under these conditions for 1 month. Alternatively, prepare small aliquots and store at -20°C for up to two years. Avoid repeated freezing and thawing cycles. | | Storage and Stability | Stable at RT for 1 week | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
