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You are here:Home » Molecular Biology » MB-BioSep Protein A/G/L » Purification Media, BioSeparation™ Affinity, Protein A, Recombinant

Purification Media, BioSeparation™ Affinity, Protein A, Recombinant

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Specifications

Recombinant Protein-A used also in Protein A beads (P9100-75) is a genetically engineered form of Protein-A derived from Staphylococcus aureus. Non-essential regions have been modified while leaving the IgG binding sites intact. Protein A is been used extensively used for the isolation of IgG from several species of mammals. The affinity of Protein A to IgG's is different between species and IgG subclasses within a given species. A major known limitation of protein A is its weak binding to Mouse IgG1– a major immunoglobulin subclass in Mice. Despite its variable binding characteristics, Protein A is a popular choice for both antibodies binding in various assays and when coupled to beads, for Immunoprecipitation of -antigen complexes. Protein A binds specifically the Fc region of immunoglobulins leaving the Fab region free for binding the antigen. Hence, Protein A is extremely useful for isolating of immune complexes.
Catalog #P9100-70
Protein A Beads characteristicsSource: Recombinant protein
Molecular Weight: 23.3kD expressed in E. coli
Purity: 90% by SDS-PAGE.
Spectroscopic analysis: OD280nm/250nm=2.51
Endotoxin: 0.5EU/mg Recombinant Protein A (LAL)
Composition: Lyophilized white Powder. 10.9mg Recombinant Protein A, 0.82 mg
phosphate buffer salts.
Storage before Reconstitution Store at 4°C. Product is stable under these conditions for
one year.
Storage after Reconstitution Store at 4°C in PBS pH 7.4 added with sodium azide 0.1% (w/v) as a preservative. Product is stable under these conditions for 1 month. Alternatively, prepare small aliquots and store at -20°C for up to two years. Avoid repeated freezing and thawing cycles.
Storage and StabilityStable at RT for 1 week
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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