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Purification Media, BioSeparation™ Affinity, Protein A Beads (Agarose)
Purification Media, BioSeparation™ Affinity, Protein A Beads (Agarose)
Pricing
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| The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human. | | | Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins. | | | Catalog # | P9100-75 | | Protein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding it presents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses useful properties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation. | | Protein A Beads Specifications | | | Matrix | Sepharose CL-4B | | Coupling method | TargetLock. | | Protein A Density | 2-4mg/ml of resin | | Binding Capacity | ~4-8mg rabbit IgG per ml of resin | | Mean bead size | 40 -165um | | Bead structure | Highly cross-linked spherical agarose, 4% | | Max back pressure | | | 0.3 MPa, 3 bar | | Max. flow rate | 4 ml/min/cm2 | | Recommended flow rate | 1-3 ml/min/cm2 | | Stability of the matrix | pH 2-11 | | Storage and Stability | May be stored at 4°C. Do not freeze. Stable for at least 6 months. | | Form | Supplied as a suspension in PBS, pH 7.4, 0.1% sodium azide. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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