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You are here:Home » Molecular Biology » MB-BioSep Protein A/G/L » Purification Media, BioSeparation™ Affinity, Protein A Beads (Agarose)

Purification Media, BioSeparation™ Affinity, Protein A Beads (Agarose)

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Specifications

The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human.
Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins.
Catalog #P9100-75
Protein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding it presents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses useful properties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation.
Protein A Beads Specifications
MatrixSepharose CL-4B
Coupling method TargetLock.
Protein A Density2-4mg/ml of resin
Binding Capacity~4-8mg rabbit IgG per ml of resin
Mean bead size40 -165um
Bead structureHighly cross-linked spherical agarose, 4%
Max back pressure
0.3 MPa, 3 bar
Max. flow rate4 ml/min/cm2
Recommended flow rate1-3 ml/min/cm2
Stability of the matrixpH 2-11
Storage and StabilityMay be stored at 4°C. Do not freeze. Stable for at least 6 months.
FormSupplied as a suspension in PBS, pH 7.4, 0.1% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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