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You are here:Home » Molecular Biology » MB-BioSep Protein A/G/L » Purification Media, BioSeparation™ Affinity, Protein G

Purification Media, BioSeparation™ Affinity, Protein G

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Specifications

Recombinant Protein G used also in Protein G beads (P9100-85) is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. Protein G has been extensively used for the isolation of IgG from several species of mammals. Although binding of Protein G to IgG's is not equivalent for all IgG subclasses, Protein G possesses properties that have made it a popular choice for antibody isolation. Protein G binds immunoglobulins at the Fc region of leaving the Fab region free to bind the antigen. Hence, Protein G is extremely useful for isolating of immune complexes.
Catalog #P9100-80
SourceRecombinant protein, expressed in E. coli
Storage & StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight33kD
SourceE. coli
Purity90% by SDS-PAGE. Spectroscopic Analysis
FormSupplied as a white lyophilized powder from PBS, pH 7.4. 10.6mg Recombinant Protein G, 0.78mg phosphate buffer salts. Reconstitute with 480ul of dH2O, 0.1% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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