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Purification Media, BioSeparation™ Affinity, Protein G
Purification Media, BioSeparation™ Affinity, Protein G
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| Recombinant Protein G used also in Protein G beads (P9100-85) is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. Protein G has been extensively used for the isolation of IgG from several species of mammals. Although binding of Protein G to IgG's is not equivalent for all IgG subclasses, Protein G possesses properties that have made it a popular choice for antibody isolation. Protein G binds immunoglobulins at the Fc region of leaving the Fab region free to bind the antigen. Hence, Protein G is extremely useful for isolating of immune complexes. | | | Catalog # | P9100-80 | | Source | Recombinant protein, expressed in E. coli | | Storage & Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | | Molecular Weight | 33kD | | Source | E. coli | | Purity | 90% by SDS-PAGE. Spectroscopic Analysis | | Form | Supplied as a white lyophilized powder from PBS, pH 7.4. 10.6mg Recombinant Protein G, 0.78mg phosphate buffer salts. Reconstitute with 480ul of dH2O, 0.1% sodium azide. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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