Purification Media, BioSeparation™ Affinity, Protein G (Agarose)

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Specifications

Protein G originated from Streptococci is a monomeric protein with a molecular weight of about

50,000 kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of

immunoglobulins from Mouse, Rabbit, Goat and Human. Due to this binding ability, Protein G is

commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads, using TargetLock chemistry, in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).

Catalog #	P9100-85
Matrix	SepharoseTM CL-4B
	Coupling method: TargetLock
	Protein G Density: 3-4mg/ml of gel
	Binding Capacity:
	~4-8mg rabbit IgG per ml of beads
	Mean bead size:
	40-165um
	Bead structure:
	Highly cross-linked spherical agarose, 4%
	Max back pressure:
	0.3MPa, 3 bar
	Max. flow rates: 4ml/min/cm2
	Recommended flow rate: 1-3 ml/min/cm2
	Stability of the matrix:
	pH 2-11.
	Storage:
	Store gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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		Download Specifications for Purification Media, BioSeparation™ Affinity, Protein G (Agarose)

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