Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-BioSep Protein A/G/L » Purification Media, BioSeparation™ Affinity, Protein G (Agarose)

Purification Media, BioSeparation™ Affinity, Protein G (Agarose)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).
Catalog #P9100-85
MatrixSepharoseTM CL-4B
Coupling Method TargetLock
Protein G Density 3-4mg/ml of gel
Binding Capacity~4-8mg rabbit IgG per ml of beads
Mean Bead Size40-165um
Bead StructureHighly cross-linked spherical agarose, 4%
Max Back Pressure0.3MPa, 3 bar
Max. Flow Rates 4ml/min/cm2
Recommended Flow Rate 1-3 ml/min/cm2
Stability of the MatrixpH 2-11.
StorageStore gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links