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You are here:Home » Molecular Biology » MB-BioSep Protein A/G/L » Purification Media, BioSeparation™ Affinity, Protein G (Agarose)

Purification Media, BioSeparation™ Affinity, Protein G (Agarose)


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Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).
Catalog #P9100-85
MatrixSepharoseTM CL-4B
Coupling Method TargetLock
Protein G Density 3-4mg/ml of gel
Binding Capacity~4-8mg rabbit IgG per ml of beads
Mean Bead Size40-165um
Bead StructureHighly cross-linked spherical agarose, 4%
Max Back Pressure0.3MPa, 3 bar
Max. Flow Rates 4ml/min/cm2
Recommended Flow Rate 1-3 ml/min/cm2
Stability of the MatrixpH 2-11.
StorageStore gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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