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You are here:Home » Molecular Biology » MB-Enzymes » Proteinase K Solution 20mg/ml

Proteinase K Solution 20mg/ml

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Specifications

Proteinase K is classified as a non-specific serine protease. It is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids.
Catalog #P9101
Applications • Isolation of high molecular weight DNA • Isolation of plasmid and genomic DNA • Isolation of RNA • Inactivation of RNase and DNase activities
Advantages • Ready to use, provided in liquid form containing 40% glycerol. • Smaller contamination risk with liquid form. • Can be stored at -20°C still having the enzyme in liquid form. • Excellent stability
Notes•Proteinase K is not inactivated by metal-chelating agents like EDTA, by thiol-reactive reagents (such as monoiodoacetic acid, p-chloromercuribenzoate) or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme.
•Proteinase K is stable over a wide pH range: 4-12.5.
•Raising the temperature of the reaction from 37°C to 50°-65°C can increase the activity of Proteinase K.
•Proteinase K can be inactivated at 95°C for 10 minutes (some enzymatic activity may still remain)
•Proteinase K retains its activity in the presence of SDS (0.5-1%) or urea (1-4M).
•Depending on the application the enzyme is used in the absence or presence of 0.2-1% SDS or 1-4M urea.
Nomenclature EC 3.4.21.14
Recommended Dilution The recommended working concentration for Proteinase K is 0.05-1mg/ml. Use 3ul of a 20mg/ml solution per 1.5ml of bacterial culture.
Optimal dilutions to be determined by researcher.
SolubilityColorless to pale yellow, clear, complete
pH 7.5 ± 0.2
Specific Activity 30 units/mg
Unit DefinitionOne unit liberates 1umole of Folin positive amino acids, measured as Tyrosine, at 37°C, pH 7.5, using urea-denatured hemoglobin as the substrate.
Quality ControlNicking Activity: None Detected
RNases: None Detected
Exonucleases: None Detected
Phosphatases: None Detected
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number39450-01-6
SourceTritirachium album limber
Concentration~20mg/ml
FormSupplied as a liquid in 10mM Tris HCl, pH 7.5, 1mM calcium acetate, 40% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.4.21.64; Tritirachium album limber


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