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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kD proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kD and the normal concentrations in human plasma is approximately 1-3ng/ml because of the very fast turnover, the proenzyme concentration is ~3ug/ml.
Catalog #	P9102-29
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:4000. Strong reaction is seen in capture ELISA in combination with a biotin labeled antigen against Protein C only when using a tris-buffer containing 2.5mM CaCl2
	Western Blot: After SDS-PAGE, reacts with protein C in non-reduced form only.
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63.Ag8.653 myeloma cells with spleen cells of female CF1xBalb/c mice.
Culture Medium	RPMI 1640 with 10% fetal calf serum
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F182
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Protein C isolated from human plasma.
Specificity	Recognizes human Protein C.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.