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You are here:Home » Antibodies » Abs to Protein C » Anti -Protein C

Anti -Protein C

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
Protein C (PC) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of PC in plasma is ~4ug/ml (~60nM). A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis. Protein C is expressed as a two-chain molecule with a molecular weight of 62kD. The light chain (21kD) of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11 g-carboxyglutamic acid (gla) residues. These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The heavy chain of PC (41kD) consists of the catalytic domain and an activation peptide. Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin. The activation of PC is associated with the release of a small activation peptide (2-3kD, called Protein C peptide, or PCP) from the N-terminal of the heavy chain. Activated Protein C (APC) is a serine protease with anticoagulant activity. APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa. The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, also called Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by alpha1-antitrypsin and alpha2-macroglobulin. The inhibitory activity of PCI is stimulated approximately 10 fold by heparin.
Catalog #P9102-33D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:50,000
Western Blot: 1-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostSheep
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M sodium phosphate, 0.1M sodium chloride, 1mM EDTA, pH 6.6. No preservative added.
PurityPurified by Protein G affinity chromatography.
ImmunogenMouse Protein C.
SpecificityRecognizes mouse Protein C and mouse APC.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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