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Anti -Protein G (Immunoglobulin G Binding Protein G [Precursor], IgG Binding Protein G, spg) (Biotin)
Anti -Protein G (Immunoglobulin G Binding Protein G [Precursor], IgG Binding Protein G,
spg) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Chicken |
Affinity Purified |
E B |
|
| Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. | | | Catalog # | P9102-57M | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA: 1:5000-1:40,000 | | Western Blot: 1:1000-1:10,000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgY | | Host | Chicken | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.1% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified recombinant Protein G | | Specificity | Recognizes Protein G. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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