| PGP9.5 is a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway. This product stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES. Also stains neuroendocrine cells in human adult gut. |
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| Catalog # | P9102-70A1 |
| Applications | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry (Paraffin): 1:200. Fixation in 95% ethanol/ 5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/ethanol will require pretreatment using microwave-citrate buffer method. |
| Western Blot: 1:100- 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 10F343 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Native Protein Gene Product 9.5, from brain. |
| Specificity | Recognizes human protein gene product 9.5. Recognizes a different epitope towards the N-terminal of the protein than P9102-70A. Species Crossreactivity: rat, guinea pig and porcine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
