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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -Protein Gene Product 9.5 (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)

Anti -Protein Gene Product 9.5 (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin
thiolesterase, PARK5)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IF
Protein gene product 9.5 (PGP 9.5) is a 27kD ubiquitin-protein hydrolase, expressed in the cytoplasm of neurons and cells of the diffuse neuroendocrine system and related tumors. PGP 9.5 hydrolyzes a peptide bond at the C-terminal glycine of the proteolysis tagging protein ubiquitin, thereby generating/recycling ubiquitin monomers, essential in the ubiquitin-proteasome cytoplasmic protein degradation system. PGP 9.5 is a focus area for neurological disorders such as Alzheimerís and Parkinsonís disease and has been linked with tumor invasion and progression.
Catalog #P9102-70F
ApplicationsSuitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 1:200-1:800. Does not require antigen retrieval prior to staining paraffin sections, but heat treatment may enhance staining. Sodium citrate buffer, pH 6.0 is recommended for this purpose.
Western Blot: 1:1000-1:5000 detects a band at ~27kD in rat brain cell lysates
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Recommended Positive Control TissuesStains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the diffuse neuroendocrine system.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.02% sodium azide.
PuritySerum
ImmunogenHuman protein gene product 9.5, from brain.
SpecificityRecognizes human protein gene product 9.5 (PGP 9.5).
Species CrossreactivityGuinea pig, sheep, rat, feline, rabbit, bovine, hamster, porcine, mouse and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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