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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA)

Anti -Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
A class of kinases that regulates an array of physiological processes and includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase, protein kinase C, Akt and RSK shares a substrate specificity characterized by arginine at position 3 relative to the phosphorylated Ser or Thr (1,2). These are referred to as Arg-directed kinases or AGC-family kinases. Phospho-(Ser/Thr) PKA Substrate Antibody provides a powerful new tool for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.
Catalog #P9103-11D
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:100
ELISA: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic phospho- PKA substrate peptide.
SpecificityDetects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position. It is a useful tool in identifying new substrates of AGC family kinases, including PKA and PKC. It does not recognize the non-phosphorylated PKA substrate motif.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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