Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -Protein Kinase C, zeta (PKCz)

Anti -Protein Kinase C, zeta (PKCz)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising , I (also known as 1), II (also known as 2) and )), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation; novel (n)PKC isoforms (comprising , , (also known as PKC-L), and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+; and atypical (a)PKC isoforms, namely , and (the mouse homolog of human PKC ) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKC .
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
PKC (PKC zeta) exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.
Catalog #P9103-40Y
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.05-0.2ug/ml
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide (KLH). derived from human PKC
SpecificityRecognizes endogenous levels of total PKC protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links