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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Protein Phosphatase 1, Ser/Thr (PP1)

Protein Phosphatase 1, Ser/Thr (PP1)

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Specifications

Catalog #P9107-05
This catalytic fragment of PP1 is highly active and displays properties characteristic of type-1 phosphatases3 high specific activity with phosphorylase as substrate, relative resistance to okadaic acid (up to 50nM), and sensitivity to inhibitor-2. Sulfhydryl compounds are required to maintain activity. Phosphate, phosphoesters, and concentrated salts are inhibitory.
ApplicationsSuitable for use In vitro dephosphorylation of proteins. Other applications not tested.
Recommended DilutionIn vitro dephosphorylation:
0.1-0.5 units total PP1 per reaction. A unit releases 1nM of phosphate/min from 15uM phosphorylase at 30°C. Inhibition by 50nM microcystin-LR or 500nM okadaic acid has been demonstrated previously. The dilution buffer should be made 50% with glycerol if diluted solutions are to be stored at -20°C. The activity was determined by a malachite green based phosphatase assay.
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRabbit skeletal muscle
PurityPurified by a combination of DEAE chromatography, divalent cation activation plus trypsin digestion, and gel filtration chromatography.1 A mixture of PP1 isoforms are present, based on peptide sequencing.
Concentration~10units/100ul
FormSupplied as a liquid in 100ul of 50% glycerol, 20mM MOPS, pH 7.5, 60mM 2-mercaptoethanol, 1mM EGTA, 0.1mM MnCl2, 1mM MgCl2 and 0.1mg/ml serum albumin.
SpecificityAbsolute specificity for protein P-Ser and P-Thr compared to P-Tyr. Reported preference for sites in transcription factor,4 steroid receptor,5 ion channels, 6 and enzymes of glycogen metabolism.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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