| Catalog # | P9107-05 |
| This catalytic fragment of PP1 is highly active and displays properties characteristic of type-1 phosphatases | 3 high specific activity with phosphorylase as substrate, relative resistance to okadaic acid (up to 50nM), and sensitivity to inhibitor-2. Sulfhydryl compounds are required to maintain activity. Phosphate, phosphoesters, and concentrated salts are inhibitory. |
| Applications | Suitable for use In vitro dephosphorylation of proteins. Other applications not tested. |
| Recommended Dilution | In vitro dephosphorylation: |
| 0.1-0.5 units total PP1 per reaction. A unit releases 1nM of phosphate/min from 15uM phosphorylase at 30°C. Inhibition by 50nM microcystin-LR or 500nM okadaic acid has been demonstrated previously. The dilution buffer should be made 50% with glycerol if diluted solutions are to be stored at -20°C. The activity was determined by a malachite green based phosphatase assay. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source | Rabbit skeletal muscle |
| Purity | Purified by a combination of DEAE chromatography, divalent cation activation plus trypsin digestion, and gel filtration chromatography.1 A mixture of PP1 isoforms are present, based on peptide sequencing. |
| Concentration | ~10units/100ul |
| Form | Supplied as a liquid in 100ul of 50% glycerol, 20mM MOPS, pH 7.5, 60mM 2-mercaptoethanol, 1mM EGTA, 0.1mM MnCl2, 1mM MgCl2 and 0.1mg/ml serum albumin. |
| Specificity | Absolute specificity for protein P-Ser and P-Thr compared to P-Tyr. Reported preference for sites in transcription factor,4 steroid receptor,5 ion channels, 6 and enzymes of glycogen metabolism. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
