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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -PPP2CB (Protein Phosphatase 2, Catalytic Subunit, Beta Isozyme, PP2Abeta, PP2A-beta, PP2CB, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform)

Anti -PPP2CB (Protein Phosphatase 2, Catalytic Subunit, Beta Isozyme, PP2Abeta, PP2A-beta,
PP2CB, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
Catalog #P9107-12A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunohistochemistry (paraffin): 1:100
Optimal dilutions to be determined by the researcher.
Positive ControlNIH/3T3 Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in sterile filtered PBS, 0.08% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide peptide conjugated to KLH corresponding to the sequence NH2-Cys-Gly-Glu-Glu-Asp-Ile-Asp-THr-Arg- Lys-Ile-Asn-Asn-Ser-Phe-COOH.
SpecificityRecognizes human PP2A, B beta subunit. Minimal cross reactivity against PP2A, B gamma subunit. Species Crossreactivity: mouse, rat, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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