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Abs to Enzymes, Phosphatase
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Anti -PPM1A (Protein Phosphatase 2C alpha, PP2Ca, Protein Phosphatase 1A, Protein Phosphatase 2C Isoform alpha, PP2C-alpha, Protein Phosphatase IA, PPPM1A)
Anti -PPM1A (Protein Phosphatase 2C alpha, PP2Ca, Protein Phosphatase 1A, Protein
Phosphatase 2C Isoform alpha, PP2C-alpha, Protein Phosphatase IA, PPPM1A)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Protein phosphatase 2C alpha (PP2C-alpha) dephosphorylates proteins with some preference for phosphor-threonine over phospho-serine residues. It is insensitive to PP1 and PP2a inhibitors such as okadaic acid, but has an absolute requirement for either magnesium or manganese ions. PP2c-alpha and can be activated by unsaturated fatty acids, such as arachidonic and oleic acid. Overexpression of PP2C-alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53. Dephosphorylation of proteins in the nucleus by PP2C-aplha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADs and stress-activated proteins such as p38 and JNK. | | | Catalog # | P9107-13E | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11C45 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | E.coli-derived recombinant human PP2C-alpha (rhPP2C-alpha; aa1-324; Accession # P35813). | | Specificity | Recognizes human PP2Ca. Species Crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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