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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -PP1 beta (protein phosphatase 1, catalytic subunit, beta isoform, MGC3672, PP-1B, PPP1CD, Serine/threonine-protein Phosphatase PP1-beta catalytic subunit)

Anti -PP1 beta (protein phosphatase 1, catalytic subunit, beta isoform, MGC3672, PP-1B,
PPP1CD, Serine/threonine-protein Phosphatase PP1-beta catalytic subunit)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IP IH
Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
Catalog #P9107-15B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500 detected PP1 beta in 10ug of HeLa lysates, Jurkat lysates, RAW 264 lysates, HEK293 lysates, NIH/3T3 lysates, C2C12 lysates, PC12 lysates, A431 lysates and HepG2 lysates.
Immunohistochemistry (Paraffin): 1:100 detected PP1 beta in normal human testis. Tissue was pretreated with citrate buffer, pH 6.0.
Immunoprecipitation: 10-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.08% sodium azide.
PurityPurified
ImmunogenPurified peptide corresponding to aa318-327 from the C-terminus of human PP1 beta catalytic subunit, conjugated to KLH.
SpecificityRecognizes human PP1 beta at ~38kD. Does not cross react with other recombinant PP1 isoforms. Species Crossreactivity: Mouse, Rat, Bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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