Anti -PP1 beta (protein phosphatase 1, catalytic subunit, beta isoform, MGC3672, PP-1B,
PPP1CD, Serine/threonine-protein Phosphatase PP1-beta catalytic subunit)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
B IP IH |
|
| Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR. | | | Catalog # | P9107-15B | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:500 detected PP1 beta in 10ug of HeLa lysates, Jurkat lysates, RAW 264 lysates, HEK293 lysates, NIH/3T3 lysates, C2C12 lysates, PC12 lysates, A431 lysates and HepG2 lysates. | | Immunohistochemistry (Paraffin): 1:100 detected PP1 beta in normal human testis. Tissue was pretreated with citrate buffer, pH 6.0. | | Immunoprecipitation: 10-15ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.08% sodium azide. | | Purity | Purified | | Immunogen | Purified peptide corresponding to aa318-327 from the C-terminus of human PP1 beta catalytic subunit, conjugated to KLH. | | Specificity | Recognizes human PP1 beta at ~38kD. Does not cross react with other recombinant PP1 isoforms. Species Crossreactivity: Mouse, Rat, Bovine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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