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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » PPP1CA, Recombinant, Human, aa1-330, His-tag (Serine/Threonine-protein Phosphatase PP1-alpha Catalytic Subunit, PP-1A, PPP1A)

PPP1CA, Recombinant, Human, aa1-330, His-tag (Serine/Threonine-protein Phosphatase
PP1-alpha Catalytic Subunit, PP-1A, PPP1A)

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Specifications

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
Catalog #P9107-17N
SourceRecombinant corresponding to aa1-330 from human PPP1CA, fused to His-tag at N-terminal expressed in E.coli.
Molecular Weight~37kD
Specific Activity>3000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
AA SequenceMGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~39.7kD
SourceE.coli
Purity~80% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 50mM Tris-HCl buffer, pH 8.0, 40% glycerol, 0.15M sodium chloride, 1mM DTT, 0.1mM PMSF, 1mM MnCl2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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