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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » PPP1R14A, Recombinant, Human (Protein phosphatase 1 regulatory subunit 14A, CPI17, CPI-17, PPP1INL)

PPP1R14A, Recombinant, Human (Protein phosphatase 1 regulatory subunit 14A, CPI17, CPI-17,
PPP1INL)

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Specifications

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.
Catalog #P9107-30Z
Recombinant protein corresponding to aa1-167 of human PPP1R14A fused to a His-tag at the N-terminal expressed in E. coli (NP_150281).
SequencesMGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity90% by SDS-PAGE. Purified using conventional chromatography techniques.
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris pH 8.0, 2mM EDTA, 1mM DTT, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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