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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » PPP2R5B (protein phosphatase 2, regulatory subunit B', beta, B56B, PP2A B subunit isoform B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit isoform R5-beta, PR61B, Serine/threonine-protein phosphatase 2A 56kD regu

PPP2R5B (protein phosphatase 2, regulatory subunit B', beta, B56B, PP2A B subunit isoform
B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit
isoform R5-beta, PR61B, Serine/threonine-protein phosphatase 2A 56kD regu

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Specifications

C-QRLTPQVAASGGQS, from the C-Terminus of human PP2A-Bí (B56).
Control peptide corresponding to polyclonal antibody, Catalog#P9107-34U, goat x human.
Catalog #P9107-34U-P
PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
ApplicationsSuitable for use in ELISA. Other Applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking (see corresponding antibody P9107-34U)Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
SourceSynthetic
PurityHighly Purified
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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