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Protein Phosphatase 2C alpha, Recombinant, Human, His-tag (PP2Ca, PPM1A, Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A)
Protein Phosphatase 2C alpha, Recombinant, Human, His-tag (PP2Ca, PPM1A, Protein
Phosphatase, Mg2+/Mn2+ Dependent, 1A)
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| Protein phosphatase 2C alpha (PP2Ca) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Ca (amino acids 1-382) was cloned into an E. coli expression vector (BamHI/HindIII site). | | | Catalog # | P9107-39 | | Molecular Weight | 46.6kD (containing His tag, T7 gene 10 leader, XpressTM Epitope) | | Sequence | MRGSHHHHHH/ GMASMTGGQQ/ MGRDLYDDDD/ KDRWILMGAF/ LDKPKMEKHN/ AQGQGNGLRY/ GLSSMQGWRV/ EMEDAHTAVI/ GLPSGLESWS/ FFAVYDGHAG/ SQVAKYCCEH/ | | LLDHITNNQD/ FKGSAGAPSV/ ENVKNGIRTG/ FLEIDEHMRV/ MSEKKHGADR/ SGSTAVGVLI/ | | SPQHTYFINC/ GDSRGLLCRN/ RKVHFFTQDH/ KPSNPLEKER/ IQNAGGSVMI/ QRVNGSLAVS/ | | RALGDFDYKC/ VHGKGPTEQL/ VSPEPEVHDI/ ERSEEDDQFI/ ILACDGIWDV/ MGNEELCDFV/ | | RSRLEVTDDL/ EKVCNEVVDT/ CLYKGSRDNM/ SVILICFPNA/ PKVSPEAVKK/ EAELDKYLEC/ | | RVEEIIKKQG/ EGVPDLVHVM/ RTLASENIPS/ LPPGGELASK/ RNVIEAVYNR/ LNPYKNDDTD/ | | STSTDDMW | | Specific Acitivity | > 8000units/mg | | Unit Definition | One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37°C using 10mM of substrate. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 46.6kD | | Source | E. coli | | Purity | 95% by SDS PAGE; Soluble His-tagged fusion protein purified by conventional chromatography techniques | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM DTT, 1mM manganese chloride and 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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