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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Protein Phosphatase 4, Regulatory Subunit 3, alpha (PP4R3 alpha, PP4R3A, PPP4R3A, KIAA2010, FLFL1, MEK1 Suppressor 1, MSTP033, SMEK Homolog 1 Suppressor of Mek1 (Dictyostelium), Smek1, smk1, smk-1) (Control Peptide)

Protein Phosphatase 4, Regulatory Subunit 3, alpha (PP4R3 alpha, PP4R3A, PPP4R3A,
KIAA2010, FLFL1, MEK1 Suppressor 1, MSTP033, SMEK Homolog 1 Suppressor of Mek1
(Dictyostelium), Smek1, smk1, smk-1) (Control Peptide)

Pricing

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Specifications

C-EQQRERQDNPKLD, from the internal region of human SMEK1.
Control peptide corresponding to polyclonal antibody, Catalog#P9107-53B, goat x human.
Catalog #P9107-53B-P
Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking (see corresponding antibody P9107-53B)Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PurityHighly Purified
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS.
SpecificityRecognizes human SMEK1 at ~110kD. Species sequence homology: mouse, rat, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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