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Anti -Protein S (PROS1, PROS, Vitamin K dependent protein S)
Anti -Protein S (PROS1, PROS, Vitamin K dependent protein S)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Highly Purified |
E B Gel |
|
| Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70kD and concists of a single polypeptide chain. The concentration of protein S in human plasma is 25ug/ml, with a half-life of approximately two days. About 40% of Protein S in plasma is in a free form, whereas 60% is complexed with C4b-binding Protein (C4BP). Only the free Protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa. | | | Catalog # | P9108-05 | | Protein S is thought to function in both the coagulation and complement cascades. It has been suggested that g-carboxyglutamic acid (gla) dependent binding of protein S to negatively charged phospholipids may function to concentrate C4BP at cell surfaces following injury. | | In the coagulation system, Protein S functions as an anticoagulant cofactor protein. Activated Protein C (APC) forms a 1 | 1 stoichiometric complex with Protein S in the presence of Ca2+ and phospholipid vesicles (Kd=6x10e-9M). In the presence of Protein S, a moderate increase (3-10 fold) in the rate of factor Va and factor VIIIa inactivation by APC is observed in plasma and on the surface of unstimulated platelets. Protein S bound to C4BP does not possess APC cofactor activity. Recently, an additional binding protein which enhances the activity of Protein S has been described. Proteolytic inactivation of Protein S by thrombin has been proposed as a regulatory mechanism in this system. A single cleavage by thrombin abolishes Protein S cofactor activity by removing an NH2-terminal peptide (Mr=8000) which contains the gla domain. | | The domain structure of Protein S is similar to that of the other vitamin K-dependent coagulation factors with the exception that Protein S does not possess the catalytic triad. Protein S is a single chain protein containing 10 gla residues in the NH2-terminal domain and 4 epidermal growth factor (EGF) domains. | | Applications | Suitable for use in Western Blot and RIEP (Radioimmunoelectrophoresis). Other applications not tested. | | Recommended Dilution | Western Blot: 10ug/ml | | Optimal dilutions to be determined by the researcher. | | Extinction Coefficient | E1%, 1cm, 280nm = 14.0 | | Molecular Weight | 150kD | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~8mg/ml | | Form | Supplied as a liquid in HEPES buffered saline, pH 7.4, 50% glycerol. | | Purity | ~ 95%. Purified by ammonium sulfate precipitation followed by Ion Exchange chromatography. | | Immunogen | Human Protein S | | Specificity | Recognizes human Protein S. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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