You are here:Home
»
Antibodies
»
Abs to Serum Proteins
»
Anti -Protein S (PROS1, PROS, Vitamin K Dependent Protein S)
Anti -Protein S (PROS1, PROS, Vitamin K Dependent Protein S)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Serum |
E IP |
|
| Protein S is a vitamin K dependent plasma glycoprotein (MW 69000) which belongs to a group of coagulation inhibitors consisting itself of proteinases. It circulates in the blood in a free active form and in an inactive form bound to C4b binding protein. Protein S is also released by thrombin stimulation from platelet alpha-granules. Protein S acts a s a cofactor for activated protein C (pCa) enhancing the cleavage of factors Va and VIIIa, producing anticoagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system. Protein S looses much of its coagulant cofactor activity after cleavage by thrombin, a process which is inhibited by thrombomodulin in the presence of free calcium ions. Plasma concentration of protein S may be significantly reduced below normal adult levels (2.5mg/ml) in | | | patients with hereditary deficiency of protein S or suffering a combination of deep vein thrombosis, superficial thrombophlebitis and pulmonary embolism. Substances such as bacterial endotoxins, tumor necrosis factor and immune mediator interleukin 1 also reduce the protein S level in plasma. Oral contraceptives also depress pS activity. A reduction in plasma protein S during pregnancy usually returns to normal after parturition. | | | Catalog # | P9108-09D | | Applications | Suitable for use in ELISA, Immunoprecipitation, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. | | Recommended Dilution | Immunoelectrophoresis: 120ul against 2ul human serum | | Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wells. | | Note: Plasma samples and all assay components must contain EDTA to stabilize the protein. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2. | | Purity | Serum | | Immunogen | Human Protein S | | Specificity | Recognizes human Protein S. A single precipitin line is obtained in immunoelectrophoresis, | | bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified protein S. Also reacts with the protein S component in complexes with protein C or with C4b binding protein. No reaction is obtained with any other plasma protein. Does not crossreact with any other component of human plasma. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
