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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)

Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)

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Specifications

Full-length (aa1-436) PTP1B with N-terminal GST-tag (GenBank Accession No. M31724)
Catalog #P9109-01A
Specific Activity~2,000 pmole/min/ug. One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C
Assay Buffer50mM Hepes, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP.
ApplicationsSuitable for use in enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Other applications not tested.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months.For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight76kD
SourceE. coli
Purity55 % by SDS-PAGE
ConcentrationAs reported
FormSupplied as a liquid in 45mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4mM KCl, 10% glycerol, 18mM glutathione, and 3mM DTT
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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