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Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)
Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)
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| The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. | | | Catalog # | P9109-14 | | AA Sequence | MEMEKEFEQI/ DKSGSWAAIY/ QDIRHEASDF/ PCRVAKLPKN/ KNRNRYRDVS/ PFDHSRIKLH/ | | QEDNDYINAS/ LIKMEEAQRS/ YILTQGPLPN/ TCGHFWEMVW/ EQKSRGVVML/ NRVMEKGSLK/ | | CAQYWPQKEE/ KEMIFEDTNL/ KLTLISEDIK/ SYYTVRQLEL/ ENLTTQETRE/ ILHFHYTTWP/ | | DFGVPESPAS/ FLNFLFKVRE/ SGSLSPEHGP/ VVVHCSAGIG/ RSGTFCLADT/ CLLLMDKRKD/ | | PSSVDIKKVL/ LEMRKFRMGL/ IQTADQLRFS/ YLAVIEGAKF/ IMGDSSVQDQ/ WKELSHEDLE/ | | PPPEHIPPPP/ RPPKRILEPH/ N | | Specific Acitivity | 6,000-12,000units/mg. | | Unit Definition | One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37°C using 10mM of substrate. | | Storage and Stability | Store at -20°C or lower. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 37,312 | | Source | E. coli | | Purity | 95% by SDS PAGE; Purified by using conventional chromatography techniques. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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