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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)

Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)

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Specifications

The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector.
Catalog #P9109-14
AA SequenceMEMEKEFEQI/ DKSGSWAAIY/ QDIRHEASDF/ PCRVAKLPKN/ KNRNRYRDVS/ PFDHSRIKLH/
QEDNDYINAS/ LIKMEEAQRS/ YILTQGPLPN/ TCGHFWEMVW/ EQKSRGVVML/ NRVMEKGSLK/
CAQYWPQKEE/ KEMIFEDTNL/ KLTLISEDIK/ SYYTVRQLEL/ ENLTTQETRE/ ILHFHYTTWP/
DFGVPESPAS/ FLNFLFKVRE/ SGSLSPEHGP/ VVVHCSAGIG/ RSGTFCLADT/ CLLLMDKRKD/
PSSVDIKKVL/ LEMRKFRMGL/ IQTADQLRFS/ YLAVIEGAKF/ IMGDSSVQDQ/ WKELSHEDLE/
PPPEHIPPPP/ RPPKRILEPH/ N
Specific Acitivity6,000-12,000units/mg.
Unit DefinitionOne unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37°C using 10mM of substrate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight37,312
SourceE. coli
Purity95% by SDS PAGE; Purified by using conventional chromatography techniques.
Concentration~1mg/ml
FormSupplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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