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You are here:Home » Antibodies » Abs to Cadherin » Anti -Protocadherin 15 (PCDH-15, PCDH15, Protocadherin-15)

Anti -Protocadherin 15 (PCDH-15, PCDH15, Protocadherin-15)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IC
Protocadherin 15 is a 250-270kD "other" member of the nonclustered group of the protocadherin family of molecules. In the adult, it is expressed on the base of hair cell stereoclila in the Organ of Corti, on cerebellar granule cells, and on rod and cone photoreceptors. In the ear, it is expressed as a homodimer, and binds (in trans) to cadherin 23 homodimers on adjacent stereocilia. Mature human PCDH-15 is a type I transmembrane protein that is 1929aa in length. It contains a 1350aa extracellular domain aa27-1376 plus a 558aa cytoplasmic region. There are 11 cadherin domains in the ECD aa40-1259. Multiple splice forms are reported. One is a soluble 110kD form that shows a five aa substitution for aa957-1955. Another contains a seven aa substitution for aa1119-1125. Others show substitutions or deletions in either the extracellular or cytoplasmic domains. Over aa27-1376, human PCDH-15 shares 95% aa identity with mouse PCDH-15.
Catalog #P9119-50C
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 5-15ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa27-376 from human PCDH15 expressed in CHO cell line.
SpecificityRecognizes human Protocadherin-15. Species sequence homology: Recombinant human Protocadherin-1, rhProtocadherin-8, and rhProtocadherin-10 (1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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