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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -Prox1 (Prospero-related Homeobox 1)

Anti -Prox1 (Prospero-related Homeobox 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #P9130
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000-1:4000 detects Prox1 in RIPA lysates from mouse liver cells. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Prox1 (1:4000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.05% sodium azide and 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide (CQSVSPRESYRENKRKQKLPQ)
corresponding to amino acids 196-215 of human prospero-related
homeobox 1 (Prox1) with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in mouse, and has 18/20 identical amino acids in chicken.
SpecificityRecognizes Prox1, Mr ~95kD. Nonspecific proteins were also detected, Mr ~47kD and Mr ~38kD. Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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