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You are here:Home » Antibodies » Abs to Ligands » Anti -P-Selectin, Glycoprotein Ligand-1 (PSGL 1)

Anti -P-Selectin, Glycoprotein Ligand-1 (PSGL 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IH IC FC
Catalog #P9162-03
ApplicationsSuitable for use in Immunohistochemistry, Flow Cytometry, Western Blot, ELISA, Immunocytochemistry and Functional Studies. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin sections): 2ug/ml
Flow Cytometry: 10ug/ml per 10e6 cells
Western Blot: 2ug/ml
Immunocytochemistry (Cell smears): 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6D712
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman P Selectin Glycoprotein Ligand-1 (PSGL 1). Epitope: aa49-62; Overlaps the three potential tyrosine sulfation sites at residues 46, 48 and 51 of the mature PSGL 1, a membrane-distal epitope on PSGL 1.
SpecificityRecognizes human PSGL 1. Blocks binding of PSGL 1 to P-selectin and L-selectin. Recognizes a membrane-distal epitope on PSGL 1 and blocks binding of (i) PSGL 1 to immobilzed P-selectin, (ii) fluid-phase P-selectin to leukocytes, and (iii) neutrophils to P-selectin under both static and a range of shear stress conditions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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