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Anti -P Selectin, Glycoprotein Ligand-1 (PSGL 1)
Anti -P Selectin, Glycoprotein Ligand-1 (PSGL 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
E B IH IC FC |
|
| Catalog # | P9162-03 | | Applications | Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot, ELISA, Immunocytochemistry and Functional Studies. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Paraffin sections): 2ug/ml | | Flow Cytometry: 10ug/ml per 10e6 cells | | Western Blot: 2ug/ml | | Immunocytochemistry (Cell smears): 2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6D712 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, before the addition of glycerol to 40%. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Human P Selectin Glycoprotein Ligand-1 (PSGL 1). Epitope: aa49-62; Overlaps the three potential tyrosine sulfation sites at residues 46, 48 and 51 of the mature PSGL 1, a membrane-distal epitope on PSGL 1. | | Specificity | Recognizes human PSGL 1. Blocks binding of PSGL 1 to P-selectin and L-selectin. Recognizes a membrane-distal epitope on PSGL 1 and blocks binding of (i) PSGL 1 to immobilzed P-selectin, (ii) fluid-phase P-selectin to leukocytes, and (iii) neutrophils to P-selectin under both static and a range of shear stress conditions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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